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Key Documents

CMC0014

Sigma-Aldrich

BL21(DE3)化学感受态细胞

Escherichia coli, rod shaped

同義詞:

Competent E. coli cells

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.85

product name

BL21(DE3)化学感受态细胞, for protein expression

生物源

Escherichia coli

等級

for molecular biology

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

BL21(DE3)化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因,可用于含有T7启动子的任何表达质粒。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

應用

大肠杆菌 BL21(DE3)菌株已用于:以pET19b作为表达载体,表达tau 2N4R同种型(aa1–441)和截短的tau(aa1–421)。
  • BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。
  • 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。
  • 特點和優勢

    • 转化效率> 1×107 CFU /µg
    • 实惠的价格
    • 可为日常蛋白表达工作提供卓越价值。

    成分

    • BL21(DE3)化学感受态细胞
    • pUC19转化对照DNA
    • 表达恢复培养基

    儲存類別代碼

    10 - Combustible liquids


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