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70954

Sigma-Aldrich

Rosetta(DE3)感受态细胞 - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

同義詞:

BL21 derivatives

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About This Item

分類程式碼代碼:
41106202

生物源

Escherichia coli

品質等級

製造商/商標名

Novagen®

儲存條件

OK to freeze

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

運輸包裝

wet ice

儲存溫度

−70°C

一般說明

基因型:F-ompT hsdSB(rB- mB-) gal dcm(DE3) pRARE(CamR)





该产品包含转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明(EUD)表格。
Rosetta宿主菌株是BL21衍生物,旨在增强真核蛋白的表达,该蛋白含有在大肠杆菌中很少使用的密码子。这些菌株在兼容的氯霉素抗性质粒上为AGG、AGA、AUA、CUA、CCC、GGA密码子提供tRNA。因此,Rosetta菌株提供了“通用”翻译,否则该翻译受大肠杆菌密码子使用的限制。tRNA基因由其天然启动子驱动。在Rosetta(DE3)pLysS中,稀有tRNA基因存在于携带T7溶菌酶基因的相同质粒上。

DE3表明宿主是λDE3的溶原菌,因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。这样的菌株适用于通过用IPTG诱导从克隆在pET载体中的靶基因生产蛋白质。
Rosetta宿主菌株是BL21衍生物,旨在增强真核蛋白的表达,该蛋白含有在大肠杆菌中很少使用的密码子。

成分

0.4 ml1 ml组分

•2 × 0.2 ml5 × 0.2 mlRosetta(DE3)感受态细胞

•2 × 2 ml4 × 2 mlSOC培养基

•2 ng2 ng供试质粒

警告

毒性:多个毒性值,请参阅MSDS(O)

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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