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CMC0017

Sigma-Aldrich

过表达 ™ C41 (DE3) 化学感受态细胞

for the highest protein expression

同義詞:

C41 strain

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.85

生物源

Escherichia coli

等級

for molecular biology

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

相關類別

一般說明

OverExpress™ 电感受态和化学感受态细胞是可以有效表达所有种类生物体（包括真细菌、酵母、植物、病毒和哺乳动物）的有毒蛋白质的大肠杆菌。这些新菌株表达有毒蛋白质的有效性已在350多篇论文中得到验证。OverExpress™菌株含有从表型上选择的基因突变，以产生对有毒蛋白质的耐受性。C41(DE3) 菌株来源于 BL21(DE3)。该菌株至少有一处突变，其防止了与许多重组有毒蛋白表达相关的细胞死亡。菌株C43（DE3）来源于C41（DE3），是通过选择对不同毒性蛋白的抗性而得到的，并且可以表达一组不同的C41（DE3）的毒性蛋白。OverExpress C41（DE3）pLysS和C43（DE3）pLysS还带有抗氯霉素的质粒，该质粒可编码T7溶菌酶，其是T7 RNA聚合酶的一种天然抑制剂。含有pLysS的细胞会产生少量的T7溶菌酶。这些菌株用于抑制诱导以前T7 RNA聚合酶的基础表达，从而使编码特别有毒的蛋白质的重组体保持稳定。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

應用

过表达 ™ C41 (DE3) 化学感受态细胞已用于表达筛选。

特點和優勢

  • 用这些 BL21 (DE3) 衍生物克隆到任何 T7 载体的表达基因
  • 有效表示毒性和膜蛋白类
  • 被超过 350 篇研究文章引用

成分

  • 过表达 C41 (DE3) 化学感受态细胞
  • pUC 19 转化对照 DNA
  • 表达回收培养基


法律資訊

OverExpress is a trademark of Imaxio, S.A.

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

分析證明 (COA)

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71397

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條款

OverExpress™ Chemically Competent Cells

Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024

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