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Merck
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重要文件

L2542

Sigma-Aldrich

LB 肉汤

Liquid microbial growth medium

同義詞:

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

等級

for molecular biology

無菌

sterile; 0.2 μm filtered

形狀

liquid

技術

microbiological culture: suitable

pH值

6.5-7.2

應用

microbiology

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

一般說明

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

應用

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

特點和優勢

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

成分

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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D O Chaffin et al.
Journal of bacteriology, 187(13), 4615-4626 (2005-06-22)
Several bacterial pathogens have evolved the means to escape immune detection by mimicking host cell surface carbohydrates that are crucial for self/non-self recognition. Sialic acid, a terminal residue on these carbohydrates, inhibits activation of the alternate pathway of complement by
Thomas P Burg et al.
Nature, 446(7139), 1066-1069 (2007-04-27)
Nanomechanical resonators enable the measurement of mass with extraordinary sensitivity. Previously, samples as light as 7 zeptograms (1 zg = 10(-21) g) have been weighed in vacuum, and proton-level resolution seems to be within reach. Resolving small mass changes requires
Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50
Antonio Basanta, Beatriz Gomez-Sala, et al.
Biotechnology, 76, 3314-3324 (2010)
Philippe M Fortune et al.
American journal of botany, 95(4), 454-464 (2008-04-01)
The origin of polyploid Bromus species of section Genea was investigated using molecular data. This group of annual species native from the Old-World is composed of three diploids, two tetraploids, one hexaploid, and one octoploid. Molecular cloning, sequencing, and phylogenetic
Felix Wezel et al.
International journal of molecular sciences, 22(6) (2021-04-04)
Invasive urothelial carcinomas of the bladder (UCB) characteristically show a loss of differentiation markers. The transcription factor Grainyhead-like 3 (GRHL3) plays an important role in the development and differentiation of normal urothelium. The contribution to UCB progression is still elusive.

文章

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

條款

General protocols for growth of competent cells in microbial medium.

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