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Merck
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重要文件

L3397

Sigma-Aldrich

LB Broth (Luria low salt)

Powder microbial growth medium

同義詞:

Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

等級

Molecular Biology
for molecular biology

品質等級

無菌

non-sterile

形狀

powder

成份

NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

技術

microbiological culture: suitable

pH值

6.8-7.2(1.55% solution)

適合性

suitable for molecular biology
nonselective for Escherichia coli
nonselective for coliforms

應用

food and beverages
microbiology

儲存溫度

room temp

一般說明

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

應用

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

特點和優勢

Luria′s low salt LB powder provides:
  • Standard formulation
  • A budget-friendly alternative to liquid
  • Easy scale-up using larger package sizes

準備報告

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

重構

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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存取文件庫

Difco Manual, 272-272 (1998)
Giorgia Manzo et al.
ACS infectious diseases, 7(8), 2310-2323 (2021-07-31)
Reliable antimicrobial susceptibility testing is essential in informing both clinical antibiotic therapy decisions and the development of new antibiotics. Mammalian cell culture media have been proposed as an alternative to bacteriological media, potentially representing some critical aspects of the infection
Michel Godin et al.
Nature methods, 7(5), 387-390 (2010-04-13)
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in
Alex Golberg et al.
AAPS PharmSciTech, 10(3), 881-886 (2009-07-03)
The purpose of this study was to examine the feasibility of using irreversible electroporation (IRE) as a non-chemical method for eliminating microorganisms of liquid drugs. The studied drug was a topical ophthalmic medication, a pharmaceutical field in which the problem
Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)

條款

General protocols for growth of competent cells in microbial medium.

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