L3272

LB Broth with agar (Luria low salt)

Powder microbial growth medium

• 同義詞: Luria Agar Base (Miller’s Modification)
• 分類程式碼代碼: 41106200
• NACRES: NA.85

屬性

• 等級: for molecular biology
• 品質等級: 200
• 無菌: non-sterile
• 形狀: powder
• 成份: Agar, 15 g/L ; NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• 技術: microbiological culture: suitable
• pH值: 6.8-7.2(3.05% solution)
• 應用: food and beverages; microbiology
• 儲存溫度: room temp
• 適合性: nonselective for Escherichia coli; nonselective for coliforms

描述

一般說明

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

應用

LB Broth with agar (Luria low salt) has been used:

• for culturing E. coli DH10B cells.
• in the isolation of diazotrophic bacteria.
• for transformation of competent E. coli strain Stbl3 for cloning.

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

特點和優勢

Luria′s low salt LB powder with agar provides:

• Easy scale-up using larger package sizes
• A budget-friendly alternative to pre-poured plates
• Standard formulation

準備報告

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

法律資訊

Zeocin is a registered trademark of Cayla Sarl

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable