推薦產品
等級
for molecular biology
品質等級
描述
TB; quick dissolve, dust-free formulation
無菌
non-sterile
形狀
powder
技術
microbiological culture: suitable
應用
agriculture
food and beverages
microbiology
儲存溫度
room temp
適合性
nonselective for Escherichia coli
nonselective for coliforms
一般說明
Terrific Broth是一个被高度引用的用于大肠杆菌培养的微生物生长培养基。 这种营养丰富的微生物肉汤含有甘油、肽、氨基酸、水溶性维生素和碳水化合物。
應用
适用于大肠杆菌菌株的非选择性培养,用于克隆、高产量生产 DNA 质粒和高产量生产重组蛋白。当添加适当的抗生素时,也适用于选择性培养。
使用了很棒的肉汤:
使用了很棒的肉汤:
- 作为在 pET28GST-LIC 中选择性生长表达 MLL SET(核原癌基因)结构域的大肠杆菌 BL21(DE3) V2RpRARE 的培养基
- 培养含有表达 IL-8(interleukin-8) 质粒的 BL21(DE3) 细胞
- 很棒的肉汤也适用于细菌悬浮液(枯草芽孢杆菌菌株 1085B)
特點和優勢
很棒的 Broth EZMix 粉末提供:
- 无尘颗粒,便于安全操作和更快混合
- 一种预算较低的液体替代品
- 改良配方(不含甘油)
- 可通过大包装轻松放大规模
成分
*请注意,某些产品规格以液体形式提供。 本品为粉末形式,并且这些规格反映了最终的重悬体积。
23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
重構
将 24.1 g 粉末和 4 mL 甘油悬浮在足量的去离子水中,制成 500 mL 溶液。 在121 °C下高温灭菌15分钟以灭菌。 先冷却再添加其他成分,如抗生素(如果需要)。
法律資訊
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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條款
General protocols for growth of competent cells in microbial medium.
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