Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

T3069

Sigma-Aldrich

Trizma® hydrochloride solution

pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

solution

concentration

2 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

8.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

Looking for similar products? Visit Product Comparison Guide

General description

Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)
Optimal DNA extractions from blood on preservation paper limits conservation genomic but not conservation genetic applications
Stowell SML, et al.
Journal for nature conservation, 46 (46)
Universal buffers for use in biochemistry and biophysical experiments
Brooke D, et al.
AIMS Biophysics, 2(3) (2)
Elke Ericson et al.
Molecular and cellular endocrinology, 412, 257-264 (2015-05-20)
In a world with increasing incidences of obesity, it becomes critical to understand the detailed regulation of appetite. To identify novel regulators of the signaling mediated by one of the key hormones of energy homeostasis, leptin, we screened a set

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service