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S6147

Sigma-Aldrich

Sephadex® G-100

BioReagent, for molecular biology, DNA grade, medium

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
23151817
NACRES:
NA.52

grade

DNA grade
for molecular biology

Quality Level

product line

BioReagent

form

beads

technique(s)

buffer exchange: suitable

matrix active group

phase

swelling

1 g swells to 9-11 mL

bead diameter

40-120 μm

pore size

exclusion limit (25 bp dsDNA; (rA)27)

pH

2-10

application(s)

life science and biopharma

compatibility

Cytiva

foreign activity

DNase, RNase, none detected

storage temp.

room temp

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General description

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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