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Key Documents

S2015

Sigma-Aldrich

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Synonym(s):

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

sterility

0.2 μm filtered

form

solution

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Components

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Related product

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.
Pierce SE, et al.
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Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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