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RNAINH

Sigma-Aldrich

Murine RNase Inhibitor

suitable for cDNA synthesis, suitable for RT-PCR

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.54

form

liquid

Quality Level

concentration

40 unit/μL

technique(s)

PCR: suitable
RT-PCR: suitable
cDNA synthesis: suitable
qPCR: suitable

shipped in

wet ice

storage temp.

−20°C

General description

Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form that can extensively inhibit various RNases (RNase A, B, C).
Compared with human-derived RNase inhibitors, mouse-derived RNase inhibitors do not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (such as qPCR).
This product is DNase, RNase and Nickase free.

Application

Murine RNase Inhibitor is Suitable for use in:
  • in vitro inhibition of ribonucleases.
  • cDNA synthesis.
  • RT-PCR.
  • in vitro transcription and translation.

Features and Benefits

Murine RNase Inhibitor has the following advantages:

RT-PCR/qPCR Compatibility:
  • Enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions.
  • High-purity E. coli soluble expression.
  • No RNase residue.

Storage Class

12 - Non Combustible Liquids

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