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P1415

Sigma-Aldrich

Prehybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

Quality Level

200

form

liquid (2x concentrate)

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

General description

Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.

Application

Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.

Components

Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
  • 200 μg/mL DNA
  • 2mg Bovine Serum Albumin (BSA)
  • 2mg Ficoll
  • 2mg Polyvinylpyrrolidone (PVP)
  • 0.15 M sodium citrate
  • 1.5M NaCl

Related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Elizabeth S Colombo et al.
Investigative ophthalmology & visual science, 48(4), 1793-1800 (2007-03-29)
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GABAergic interneurons represent a heterogenous group of cell types in neocortex that can be clustered based on developmental origin, morphology, physiology, and connectivity. Two abundant populations of cortical GABAergic interneurons include the low-threshold, somatostatin (SST)-expressing cells and the fast-spiking, parvalbumin

Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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