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M6774

Sigma-Aldrich

McCown′s Woody Plant Basal Salt Mixture

powder, suitable for plant cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

General description

McCown′s Woody Plant Basal Salt Mixture is the non-vitamin component (chemstock) of the Woody Plant Medium (WPM) is used for propagation and elongation of root cultures.

Application

McCown′s Woody Plant Basal Salt Mixture has been used to culture apical and axillary buds. It has also been used to subculture individual shoots for the establishment of shoot tips in tissue culture.

Formula variant

With the macro- and micronutrients as described by Lloyd and McCown (1981).

Media Formulation

Quantity

Formulated to contain 2.3 grams of powder per liter of medium.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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E T H Franco et al.
Brazilian journal of biology = Revista brasleira de biologia, 66(2A), 455-462 (2006-07-25)
The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first
Siriwan Phongprueksapattana et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(9-10), 691-698 (2008-12-02)
Hairy root cultures of Mitragyna speciosa were established by infection of Agrobacterium rhizogenes ATCC 15834 and maintained in McCown woody plant medium (WPM) supplemented with 0.5 mg/1 naphthaleneacetic acid. The hairy roots were identified for the rooting genes loci of
Causative role of grapevine red blotch virus in red blotch disease
Yepes LM, et al.
Phytopathology, 108(7), 902-909 (2018)
Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture.
Lloyd, G., and McCown, B.H.
Proc. Int. Plant Prop. Soc., 30, 421-427 (1981)
Enhanced micropropagation and establishment of grapevine rootstock genotypes
Alzubi H, et al.
International Journal of Plant Developmental Biology, 6(1), 9-14 (2012)
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Articles

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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