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Key Documents

D9885

Sigma-Aldrich

DEAE-Dextran hydrochloride

powder

Synonym(s):

Diethylaminoethyl-dextran hydrochloride

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About This Item

CAS Number:
9064-91-9
MDL number:
MFCD00081561
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

bacterial (Leuconostoc mesenteroides)

Quality Level

200

form

powder

extent of labeling

~3.2% nitrogen

useful pH range

4.0 - 6.0

solubility

H2O: soluble 100 mg/mL, clear, colorless to faintly yellow

storage temp.

room temp

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Application

DEAE-Dextran 500, a polycationic stabilizing molecule and transfection reagent, is used in the development of sustained release protein delivery and nucleic acid transfection systems. DEAE-Dextran 500 may be used for cell immobilization in biosensors.

Preparation Note

Prepared from dextran of average mol. wt. 500,000

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Anita Bogdanov et al.
Antimicrobial agents and chemotherapy, 58(1), 405-413 (2013-11-06)
Chlamydiae are obligate intracellular bacteria that propagate in the inclusion, a specific niche inside the host cell. The standard method for counting chlamydiae is immunofluorescent staining and manual counting of chlamydial inclusions. High- or medium-throughput estimation of the reduction in
Transfection using DEAE-dextran.
Selden RF.
Current Protocols in Immunology, 10, 10-10 (2001)
Transfection using DEAE-dextran.
Gulick T.
Current Protocols in Cell Biology, 20, 20-20 (2003)
DEAE-dextran transfection of mammalian cultured cells.
E T Schenborn et al.
Methods in molecular biology (Clifton, N.J.), 130, 147-153 (1999-12-10)
J Zarate et al.
Journal of microencapsulation, 28(7), 614-620 (2011-07-12)
Bovine serum albumin (BSA) loaded calcium alginate microparticles (MPs) produced in this study by a w/o emulsification and external gelation method exhibited spherical and fairly smooth and porous morphology with 1.052 ± 0.057 µm modal particle size. The high permeability of the calcium
View All Related Papers

Related Content

Dextran and Related Polysaccharides

Learn about C6H10O6 or dextran formation, classes and naming from MilliporeSigma. Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, which have a linear backbone of α-linked d-glucopyranosyl repeating units.

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