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CMC0008

Sigma-Aldrich

STEADY Chemically Competent Cells

Escherichia coli, rod shaped

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About This Item

UNSPSC Code:
12352200

product name

STEADY Chemically Competent Cells, for plasmid production using unstable DNA

biological source

Escherichia coli

grade

for molecular biology

form

buffered aqueous solution

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: >1 x 108 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Features and Benefits

  • Clone unstable sequences with reliable results
  • Available as chemically competent or electrocompetent cells
  • Your choice of two packaging formats
  • High efficiency: over 1 × 108 cfu/μg (chemi) or 1 × 1010 cfu/μg (electro)
  • Excellent value

Components

  • STEADY chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Bacterial Transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.

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