77618

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 49.5:49.5:1

• Synonym(s): phenol:Chloroform:Isoamyl Alcohol
• CAS Number: 136112-00-0
• MDL number: MFCD00133763
• UNSPSC Code: 12352401
• PubChem Substance ID: 329767817
• NACRES: NA.07

Properties

• grade: for molecular biology
• Quality Level: 100
• product line: BioUltra
• form: liquid
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 7.7-8.3(H₂O-phase, after extraction with H₂O, 1:1)
• density: 1.29 g/mL at 20 °C
• absorption: cut-off at 360 nm
• storage temp.: 2-8°C
• SMILES string: ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
• InChI: 1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
• InChI key: ZYWFEOZQIUMEGL-UHFFFAOYSA-N

Description

Application

• Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022).


• Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018).


• The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015).


• The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013).

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 49.5:49.5:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Other Notes

Large- and small-scale phenol extractions

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301,H312 + H332,H314,H336,H341,H351,H361d,H372,H373,H411
• pcodes: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
• target_organs: Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3
• flash_point_f: 177.8 °F - closed cup
• flash_point_c: 81 °C - closed cup
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter