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P4682

Sigma-Aldrich

Phenol solution

Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology

Synonym(s):
nucleic acid extraction
Empirical Formula (Hill Notation):
C6H6O
CAS Number:
Molecular Weight:
94.11
Beilstein:
6428463
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

form

liquid

technique(s)

RNA purification: suitable

pH

4.1-4.5 (25 °C)

storage temp.

2-8°C

SMILES string

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

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40063P455746344
Phenol solution Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology

Sigma-Aldrich

P4682

Phenol solution

Phenol solution 5000 μg/mL in methanol, certified reference material

Supelco

40063

Phenol solution

Phenol solution Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Sigma-Aldrich

P4557

Phenol solution

Phenol solution 100 μg/mL in acetonitrile, PESTANAL®, analytical standard

Supelco

46344

Phenol solution

grade

for molecular biology

grade

certified reference material, TraceCERT®

grade

for molecular biology

grade

analytical standard

form

liquid

form

-

form

liquid

form

-

technique(s)

RNA purification: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

pH

4.1-4.5 (25 °C)

pH

-

pH

7.7-8.1 (25 °C)

pH

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

product line

BioReagent

product line

TraceCERT®

product line

BioReagent

product line

PESTANAL®

General description

Phenol solution is a clear liquid saturated with citrate buffer. Phenols are organic compounds that feature a hydroxyl group directly bonded to an aromatic carbocyclic ring. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

  • for the extraction of RNA by standard phenol-chloroform protocols
  • in cell wall monosaccharide analysis to treat epidermal strips from the abaxial side of Commelina communisin leaves
  • as a component of lysis-denaturation buffer for RNA isolation

Biochem/physiol Actions

Phenol solution can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols. This organic solvent also serves as a potential protein denaturant. Phenol-chloroform extraction is a broadly used method for the extraction of nucleic acids. Despite phenol′s incomplete inhibition of RNase activity, it possesses characteristics that make it useful for isolating nucleic acids when combined with chloroform and alcohol.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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