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71196

Sigma-Aldrich

Sodium acetate solution

BioUltra, for molecular biology, ~3 M in H2O

Empirical Formula (Hill Notation):
C2H3NaO2
CAS Number:
Molecular Weight:
82.03
Beilstein:
3595639
MDL number:
eCl@ss:
39021906
PubChem Substance ID:

Quality Level

grade

for molecular biology

product line

BioUltra

form

liquid

concentration

~3 M in H2O

technique(s)

precipitation assay: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

7.0-8.0 (25 °C, 3 M in H2O)

anion traces

chloride (Cl-): ≤10 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

3 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.030
λ: 280 nm Amax: ≤0.024

SMILES string

[Na+].CC([O-])=O

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

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Application

Sodium acetate solution has been used:
  • in DNA extraction
  • in reverse crosslinking and DNA purification
  • to precipitate Gata6 (GATA binding protein 6) cDNA as a part of high-purity plasmid preparation

Biochem/physiol Actions

Sodium acetate serves as a fixed base. It is useful to rectify acidosis among acidotic patients. Orginally, sodium acetate is used as dialysate for hemodialysis, but is known to cause cardiovascular instability.

Other Notes

Basic method of DNA precipitation with ethanol

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

More documents

Quotes and Ordering

D.M. Wallace
Methods in Enzymology, 152, 42-42 (1987)
An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations
Brind Amour J, et al.
Nature Communications, 6, 6033-6033 (2015)
Genome-wide mapping of DNase I hypersensitive sites in plants
Plant Functional Genomics, 71-89 (2015)
Sodium acetate infusion in critically ill trauma patients for hyperchloremic acidosis
McCague A, et al.
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 19(1), 24-24 (2011)
Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells
Niakan KK, et al.
Nature Protocols, 8(6), 1028-1028 (2013)

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