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77607

Sigma-Aldrich

Phenol

BioUltra, for molecular biology, TE-saturated, ~73% (T)

Synonym(s):
Hydroxybenzene
Linear Formula:
C6H5OH
CAS Number:
Molecular Weight:
94.11
Beilstein:
969616
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

for molecular biology

vapor density

3.24 (vs air)

vapor pressure

0.09 psi ( 55 °C)
0.36 mmHg ( 20 °C)

product line

BioUltra

assay

~73% (T)

form

liquid

autoignition temp.

1319 °F

quality

TE-saturated

contains

~0.1% 8-hydroxyquinoline as preservative

expl. lim.

8.6 %

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.48

pH

~7.8(aqueous phase)

bp

182 °C (lit.)

mp

40-42 °C (lit.)

density

1.071 g/mL at 25 °C (lit.)

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

Oc1ccccc1

suitability

suitable for molecular biology

storage temp.

2-8°C

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

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General description

saturated with 10 mM TRIS buffer pH 8.0 and 1 mM EDTA
Phenol, also known as hydroxybenzene, is an aromatic compound produced both naturally and synthetically. It acts as a disinfectant and is highly toxic. Exposure to phenol through inhalation, cutaneous adsorption, or oral ingestion causes denaturation and precipitation of cellular protein, which in turn leads to poisoning.

Application

Phenol (TE-saturated) has been used in the phenol-chloroform extraction procedure for plasmid DNA isolation. It has also been used to extract the aqueous phase during DNA linearization and mRNA synthesis.

Caution

according to temperature, a small quantity of an overlaid aqueous phase may form

Other Notes

RNA fingerprinting - preparation of the E. coli tRNA carrier

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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Mulder J, et al.
Bio-protocol, 1-18 (2018)
A.D. Branch et al.
Methods in Enzymology, 180, 133-133 (1989)
Thitiporn Anunthawan et al.
Biochimica et biophysica acta, 1848(6), 1352-1358 (2015-03-15)
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Mark J Lee et al.
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