65497

TRIS acetate-EDTA Buffer solution

for Northern and Southern blotting, solution

• UNSPSC Code: 12161700

Properties

• grade: for molecular biology
• product line: BioReagent
• form: solution
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 8.3±0.2
• density: 1.00 g/mL at 20 °C
• storage temp.: room temp

Description

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This concentrate can be easily diluted to 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. Especially useful for dsDNA separations.

Components

The TAE liquid buffer is a 1x solution that contains 0.04M TRIS acetate and 0.001M EDTA in water.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves