F9252

Sigma-Aldrich

Folin & Ciocalteu′s phenol reagent

suitable for determination of total protein by Lowry method, 2 N

MDL number:
NACRES:
NA.47

form

liquid

Quality Level

concentration

2 N

application(s)

hematology: suitable
histology: suitable

pH

<0.5 (20 °C)

density

1.240 g/cm3 at 20 °C

suitability

suitable for determination of total protein by Lowry method

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

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General description

Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations.
In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine.
Many modifications of the original assay procedure have been published, including methods for enhancing the color development, for determining the content of insoluble proteins, and for automating the procedure. Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay.
Folin & Ciocalteu′s phenol reagent can also be used as a spray reagent in chromatographic procedures.

Application

Folin & Ciocalteu′s phenol reagent has been used for the estimation of total phenolic content in samples.

Packaging

1 L in glass bottle
100, 500 mL in glass bottle

Other Notes

Folin & Ciocalteu′s phenol reagent does not contain phenol. Rather, the reagent will react with phenols and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.

Linkage

This product is also available as part of a kit.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

RIDADR

UN 3264 8 / PGIII

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. What should Product F9252, Folin & Ciocalteu's phenol reagent, look like?

    The Folin & Ciocalteu's phenol reagent should be a clear yellow solution.  If the solution turns green, it may not be suitable and it should not be used.

  2. Does Product F9252, Folin & Ciocalteu's phenol reagent, contain phenol?

    The Folin & Ciocalteu's phenol reagent does not contain phenol but reacts with phenol and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent.
Ainsworth EA and Gillespie KM
Nature Protocols, 2, 875-875 (2007)
David L Jones et al.
PloS one, 9(3), e90882-e90882 (2014-03-19)
Monitoring the properties of dissolved organic carbon (DOC) in soil water is frequently used to evaluate changes in soil quality and to explain shifts in freshwater ecosystem functioning. Using >700 individual soils (0-15 cm) collected from a 209,331 km(2) area...
Han-Shin Kim et al.
Scientific reports, 6, 25318-25318 (2016-05-05)
Biofilm formation on biotic or abiotic surfaces has unwanted consequences in medical, clinical, and industrial settings. Treatments with antibiotics or biocides are often ineffective in eradicating biofilms. Promising alternatives to conventional agents are biofilm-inhibiting compounds regulating biofilm development without toxicity...
Bioactive compounds and antioxidant activities during fruit ripening of watermelon cultivars.
Tlili I et al.
J. Food Compos. Anal., 24, 923-928 (2011)
E Larson et al.
Analytical biochemistry, 155(2), 243-248 (1986-06-01)
Addition of dithiothreitol in the Lowry procedure 3 min after adding the Folin-Ciocalteau reagent produces immediate color development, with 35 to 60% greater absorbance per mass of protein used.
Articles
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
Read More
Protocols
To standardize a procedure for the determination of protein by modified Lowry.
Read More
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
Read More
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
Read More

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