F9252
liquid
2 N
clear yellow
<0.5 (20 °C)
1.240 g/cm3 at 20 °C
suitable for determination of total protein by Lowry method
diagnostic assay manufacturing
hematology
histology
room temp
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Danger
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
8B - Non-combustible, corrosive hazardous materials
WGK 1
Not applicable
Not applicable
Enter Lot Number to search for Certificate of Analysis (COA).
Enter Lot Number to search for Certificate of Origin (COO).
Generally, Folin & Ciocalteu's phenol reagent is used to determine protein concentrations between 1 to 100 μg/mL. However, the reagent exhibits a linear response to protein concentration up to 1000 μg/mL when used according to the instructions for kit Product No. TP0200.
If the protein has an abnormally low tyrosine content, this method would not be suitable.
The Folin & Ciocalteu's phenol reagent should be a clear yellow solution. If the solution turns green, it may not be suitable and it should not be used.
The Folin & Ciocalteu's phenol reagent does not contain phenol but reacts with phenol and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
To standardize a procedure for the determination of protein by modified Lowry.
Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases, which is what we do during our quality control procedures.
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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