cell culture | mammalian: suitable
sodium pyruvate: 0.055 g/L
phenol red: yes
glucose: 3.15 g/L
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12 - Non Combustible Liquids
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This product is a 1:1 ratio mixture of Dulbecco’s Modified Eagle’s Medium (DME) high glucose and Nutrient Mixture F-12 Ham (F-12).
This is a 1:1 mixture of the DME and F-12. The optimal carbon dioxide required for DME is 10% and for F-12 is 5%. Since this medium is a mixture, the optimal carbon dioxide concentration is typically 5% to 8%.
When DME/F12 is used under serum free conditions, supplementation is necessary. DME/F12 can be used for growing the epithelial cell line TM4 (mouse testes) when supplemented with insulin, transferrin, EGF, FSH, somatomedin C, ovine growth hormone, and retinoic acid. Primary cultures of Sertoli cells can also be grown in the above supplemented media. Rat glial C6 cells have been grown in DME/F12 supplemented with transferrin, insulin, LH, and rat submaxillary gland extract. See: Jakoby, W.B., and Pastan, I.H., Cell Culture, Methods in Enzymology, 58, 103-106 (1979). (This book is listed as Product No. C0541).
We recommend storing Product No. D8437 at 2-8°C.
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