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Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

DME/F-12, 1:1 mixture, DMEM Hams F12
MDL number:

Quality Level






cell culture | mammalian: suitable


endotoxin, tested


HEPES: yes
L-glutamine: yes
sodium pyruvate: 0.055 g/L
phenol red: yes
glucose: 3.15 g/L

shipped in


storage temp.


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Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    This product is a 1:1 ratio mixture of Dulbecco’s Modified Eagle’s Medium (DME) high glucose and Nutrient Mixture F-12 Ham (F-12).

  4. What is the optimal carbon dioxide concentration needed for Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    This is a 1:1 mixture of the DME and F-12. The optimal carbon dioxide required for DME is 10% and for F-12 is 5%. Since this medium is a mixture, the optimal carbon dioxide concentration is typically 5% to 8%.

  5. Can Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, be used under serum free conditions?

    When DME/F12 is used under serum free conditions, supplementation is necessary. DME/F12 can be used for growing the epithelial cell line TM4 (mouse testes) when supplemented with insulin, transferrin, EGF, FSH, somatomedin C, ovine growth hormone, and retinoic acid. Primary cultures of Sertoli cells can also be grown in the above supplemented media. Rat glial C6 cells have been grown in DME/F12 supplemented with transferrin, insulin, LH, and rat submaxillary gland extract. See: Jakoby, W.B., and Pastan, I.H., Cell Culture, Methods in Enzymology, 58, 103-106 (1979). (This book is listed as Product No. C0541).

  6. What is the storage temperature of Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    We recommend storing Product No. D8437 at 2-8°C.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

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