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SCM162

Sigma-Aldrich

DMEM/F-12 PLUS Basal Medium

Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.

Synonym(s):
Organoid Basal Media, Organoid Basal Media, DMEMF12, DMEM F12, Dulbecco′s Modified Eagle Medium/Ham′s F-12, Serum-Free Basal Media, Advanced DMEM/F12
eCl@ss:
32160801

form

liquid

technique(s)

cell culture | stem cell: suitable

Related Categories

General description

DMEM/F-12 PLUS is an advanced formulation of Dulbecco′s Modified Eagle Medium/Ham′s F-12 basal media that can be used in a broad array of cell lines for applications that require reduced or no serum supplementation. Cell lines cultured in DMEM/F12 PLUS and reduced serum exhibit equivalent or superior cell growth while maintaining the same cell morphology and function as in high serum conditions. Applications for DMEM/F12 PLUS include but are not limited to the following:

• Culture of primary, stem cells and cell lines in applications that require reduced or serum-free medium.
• Optimization of transfections that require reduced or serum-free conditions.
• Production of conditioned medium in reduced serum or serum-free conditions.
• Optimized media for the culture and expansion of multiple 3D organoids and tissues systems.

DMEM/F12 PLUS is a proprietary formulation that contains human serum albumin (HSA), defined lipids, trace elements, chemicals and proteins essential for optimal cell growth in reduced serum conditions. The media contains high-glucose and does not contain L-glutamine or penicillin-streptomycin.

Application

Research Category
Stem Cell Research
Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.

Quality

Appearance (color): Clear, Red Liquid
Osmolality: 290-330 mOsm
pH: 7.2-7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative

Storage and Stability

Store at 2-8°C. Before use, aliquot into smaller volumes to avoid contamination. Protect from light when not in use. Acclimate the medium at room temperature for 15-20 minutes before use. Do not warm media at 37°C before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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