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Key Documents

P2759

Sigma-Aldrich

Phloxine B

Powder

Synonym(s):

2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluorescein disodium salt, Acid Red 92, Cyanosine, D&C;Red No. 28, Eosin 10B

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About This Item

Empirical Formula (Hill Notation):
C20H2Br4Cl4Na2O5
CAS Number:
Molecular Weight:
829.63
Colour Index Number:
45410
Beilstein/REAXYS Number:
3887100
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Phloxine B, antibacterial fluorescent dye

Quality Level

form

powder

technique(s)

microbe id | staining: suitable

color

dark red

solubility

water: 1 mg/mL, clear, red

εmax

1150-1300 at 546-550 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(Br)cc2c(Oc3c(Br)c([O-])c(Br)cc3C24OC(=O)c5c(Cl)c(Cl)c(Cl)c(Cl)c45)c1Br

InChI

1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2

InChI key

OOYIOIOOWUGAHD-UHFFFAOYSA-L

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Application

Phloxine B has been used to trace the uptake of double stranded RNA by green peach aphids. It has also been used to stain yeast strains.

Biochem/physiol Actions

Phloxine B has been demonstrated as an alternative to Gram staining. It was found to inhibit the growth of methicillin-resistant Staphylococcus aureus. Phloxine B stimulated and inhibited the channel activity of wild-type cystic fibrosis transmembrane conductance regulator (CFTR). It has antibacterial properties. Phloxine B staining can be used to distinguish between gram-positive and gram-negative bacteria.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Advances in measuring lifespan in the yeast Saccharomyces cerevisiae
Nadege Minios
Proceedings of the National Academy of Sciences of the USA (2004)
Phloxine B, a versatile bacterial stain
Reuven Rrasooly
Pathogens and Disease (2007)
Phloxine B Interacts with the Cystic Fibrosis Transmembrane Conductance Regulator at Multiple Sites to Modulate Channel Activity*210
Zhiwei Cai and David N. Sheppard
The Journal of Biological Chemistry (2002)
Using Vital Dyes to Trace Uptake of dsRNA by Green Peach Aphid Allows Effective Assessment of Target Gene Knockdown
International Journal of Molecular Sciences (2017)
Ander M de Lecea et al.
Rapid communications in mass spectrometry : RCM, 25(13), 1853-1861 (2011-06-04)
Stable isotope measurements are an important tool for ecosystem trophic linkage studies. Ideally, fresh samples should be used for isotopic analysis, but in many cases organisms must be preserved and analysed later. In some cases dyes must be used to

Protocols

Separation of Tartrazine; Amaranth; Indigo carmine; New Coccine; Sunset Yellow FCF; Allura Red AC; Fast Green FCF; Erioglaucine disodium salt; Erythrosin B sodium salt; Phloxine B; Rose bengal

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