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MAK117

Sigma-Aldrich

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Synonym(s):

Glycerin Assay Kit, Glycerine Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 colorimetric or fluorometric tests

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−20°C

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Principle

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids


Certificates of Analysis (COA)

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Paula Dunayevich et al.
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Cells make decisions based on a combination of external and internal signals. In yeast, the high osmolarity response (HOG) is a mitogen-activated protein kinase (MAPK) pathway that responds to a variety of stimuli, and it is central to the general
Sylvia J Gasparini et al.
Diabetologia, 62(8), 1463-1477 (2019-05-18)
Chronic glucocorticoid therapy causes insulin resistance, dyslipidaemia, abnormal fat accumulation, loss of muscle mass and osteoporosis. Here we describe a hitherto unknown sexual dimorphism in the metabolic response to chronic glucocorticoid exposure in mice. This led us to investigate whether

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