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Rose bengal

Dye content 95 %

Acid Red 94, Bengal Rose B sodium salt, 4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt, Rose Bengal sodium salt
Empirical Formula (Hill Notation):
CAS Number:
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Quality Level




Dye content, 95%


549 nm


diagnostic assay manufacturing

storage temp.

room temp

SMILES string




InChI key


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General description

Rose Bengal is a red or rose color dye and is anionic in nature. It is a 4,5,6,7-tetrachloro 2′,4′,5′,7′-tetraiodo derivative of fluorescein. It is widely used to detect damage to the ocular surface epithelium in ocular surface diseases such as dry eye and herpetic keratitis. Rose Bengal has mild antiviral potency.
Rose Bengal is used to stain dead and degenerating cells and mucus.


Rose Bengal has been used to evaluate acrosome integrity. It has also been used to initiate photothrombotic occlusion in blood.


1, 5 g in glass bottle

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Rose Bengal, Product 330000?

    According to The Sigma-Aldrich Handbook of Stains, Dyes and Indicators, Rose Bengal is soluble in water at 100 mg/mL, ethanol at 30 mg/mL and 2-methoxyethanol at 60 mg/mL. Conn's Biological Stains (10th Edition) states Rose Bengal has a solubility of 36% in water and 8% in ethanol.

  6. What types of applications can Product 330000, Rose bengal be used for?

    According to the literature, Rose bengal has been used in a used in neutrophil adherence test and as a generator of singlet oxygen when exposed to laser energy. See: McKinnon, J. , Expt. Med., 164, 1864, (1986) and Gamble and Vadas, Science, 242, 97, (1988).

  7. What is the quantum yield of Rose bengal, Product 330000?

    According to the literature, the quantum yield of Rose bengal is 0.11. See: Seybold, P.G., et al., Calorimetric, photometric and lifetime determinations of fluorescence yields of fluorescein dyes, Photochem. Photobiol., 9, 229-242 (1969).

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HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

Separation of Tartrazine; Amaranth; Indigo carmine; New Coccine; Sunset Yellow FCF; Allura Red AC; Fast Green FCF; Erioglaucine disodium salt; Erythrosin B sodium salt; Phloxine B; Rose bengal

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