All Photos(1)

198269

Sigma-Aldrich

Erythrosin B

certified by the Biological Stain Commission, Dye content 90 %

Synonym(s):
Iodoeosin, 2′,4′,5′,7′-Tetraiodofluorescein disodium salt, Acid Red 51, Erythrosin extra bluish
Empirical Formula (Hill Notation):
C20H6I4Na2O5
CAS Number:
Molecular Weight:
879.86
Colour Index Number:
45430
Beilstein:
1443945
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, 90%

pH

6.4 (30.3 °C)

mp

315 °C

solubility

H2O: 1 mg/mL, clear

λmax

525 nm

ε (extinction coefficient)

≥13000 at 308-312 nm
≥32000 at 259-263 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(I)cc2c(Oc3c(I)c([O-])c(I)cc3C24OC(=O)c5ccccc45)c1I

InChI

1S/C20H8I4O5.2Na/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20;;/h1-6,25-26H;;/q;2*+1/p-2

InChI key

RAGZEDHHTPQLAI-UHFFFAOYSA-L

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Application

Erythrosin B has been used as a dye in fluorescence lifetime imaging.

Packaging

25 g in glass bottle

Biochem/physiol Actions

Erythrosin B, also called as eosin B, is a xanthene dye. It belongs to the family of fluorescein dyes. Erythrosin B is an artificial food dye that induces hyperkinesis when swallowed by susceptible children. It plays a major role in inhibiting dopamine uptake and high affinity 3H-ouabain binding and ion transport in synaptosomes from rat caudate nucleus.

Suitability

Counterstain to alum-hematoxylin.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

359.6 °F - closed cup

Flash Point(C)

182 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Arch. Anat. Physiol., 396-396 (1895)
Bright monomeric red fluorescent protein with an extended
fluorescence lifetime
Merzlyak EM
Nature Methods, 4(7), 555-557 (2007)
Erythrosin B inhibits dopamine transport in rat caudate synaptosomes.
Lafferman JA and Silbergeld EK.
Science, 205(4404), 410-412 (1979)
P Johnson et al.
The Biochemical journal, 203(1), 313-321 (1982-04-01)
We have previously described a method for measuring the rotational diffusion of membrane proteins by using fluorescent triplet probes [Johnson & Garland (1981) FEBS Lett. 135, 252-256]. We now describe the criteria by which the suitability of such probes may
C. R. Acad. Sci. (D), 179, 367-367 (1924)

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