5.08605

Sodium Orthovanadate, Activated

• Synonym(s): Sodium Orthovanadate, Activated, Orthovanadate, Activated, Vanadate, Activated
• Empirical Formula (Hill Notation): Na₃O₄V
• Molecular Weight: 183.91
• UNSPSC Code: 12352202
• NACRES: NB.21

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• color: colorless
• storage temp.: −70°C

Description

General description

A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.

Biochem/physiol Actions

Primary Target
Phosphatases

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Toxic (F)

Physical form

Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Other Notes

Gordon, J.A. 1984. Methods. Enzymol.201, 477.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany