71736

Sigma-Aldrich

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H2O

Synonym(s):
Sodium lauryl sulfate solution, Lauryl sulfate sodium salt, SDS
Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
Molecular Weight:
288.38
Beilstein/REAXYS Number:
3599286
MDL number:
eCl@ss:
39093306
PubChem Substance ID:
NACRES:
NA.26

Quality Level

grade

for molecular biology

description

anionic

product line

BioUltra

mol wt

288.38 g/mol

concentration

10% in H2O

application(s)

HPLC: suitable
protein quantification: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.0-6.5

density

1.01 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg
phosphate (PO43-): ≤1 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.3
λ: 280 nm Amax: 0.2

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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Related Categories

Application

Sodium dodecyl sulfate solution has been used:
  • in the preparation of 2x extraction buffer
  • as a component in elution buffer for immunoprecipitation
  • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Dictyostelium cultivation, transfection, microscopy and fractionation
Hirst J, et al.
Bio-protocol, 5(11) (2015)
Construction of FWPV Chimaeric MVA
Buttigieg K R and Skinner M A
Bio-protocol, 5(1) (2015)
D.M. Wallace
Methods in Enzymology, 125, 39-39 (1987)
Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy
Yang H, et al.
Nature Protocols, 10(3), 382-382 (2015)
Resilient Ribonucleases
RNA Methodologies (2010)
Protocols
TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.
Read More

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