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71736

Sigma-Aldrich

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H2O

Synonym(s):

Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution

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About This Item

Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
151-21-3
Molecular Weight:
288.38
Beilstein/REAXYS Number:
3599286
MDL number:
MFCD00036175
UNSPSC Code:
12161902
eCl@ss:
39093306
PubChem Substance ID:
57652355
NACRES:
NA.26

grade

for molecular biology

description

anionic

product line

BioUltra

form

liquid

mol wt

288.38 g/mol

concentration

10% in H2O

technique(s)

HPLC: suitable
protein quantification: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

5.0-6.5

density

1.01 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg
phosphate (PO43-): ≤1 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.3
λ: 280 nm Amax: 0.2

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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Related Categories

Application

Sodium dodecyl sulfate solution has been used:
  • in the preparation of 2x extraction buffer
  • as a component in elution buffer for immunoprecipitation
  • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dictyostelium cultivation, transfection, microscopy and fractionation
Hirst J, et al.
Bio-protocol, 5(11) (2015)
Resilient Ribonucleases
RNA Methodologies (2010)
Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy
Yang H, et al.
Nature Protocols, 10(3), 382-382 (2015)
D.M. Wallace
Methods in Enzymology, 125, 39-39 (1987)
Porous nucleating agents for protein crystallization
Khurshid S, et al.
Nature Protocols, 9(7), 1621-1621 (2014)
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Protocols

TRI Reagent® Protocol

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

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