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Merck

HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange

HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange application for HPLC

Materiały

standardowe

Numer produktu
Opis
Cennik

Elastase from porcine pancreas

Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

Elastase from porcine pancreas

Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)

Elastase from human leukocytes

lyophilized powder, ≥50 units/mg protein (Bradford)

Elastase from porcine pancreas

Type I, ≥4.0 units/mg protein

Elastase from porcine pancreas

powder, white, ≥10 U/mg

Elastase from porcine pancreas

lyophilized, powder, white, ~8 U/mg

CONDITIONS

column

Discovery BIO PolyMA-SCX, 5cm × 4.6mm, 5μm particles (59601-U)

mobile phase

[A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0

gradient

0 to 100% B in 30 min. (linear)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μL

Opis

Komentarz do analizy

Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.

Informacje prawne

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany