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Merck

E7885

Sigma-Aldrich

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

Synonim(y):

Elastase from hog pancreas, Pancreatopeptidase E

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About This Item

Numer CAS:
Numer EC enzymu:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.75

pochodzenie biologiczne

Porcine

Postać

lyophilized powder

aktywność właściwa

≥4 units/mg protein

masa cząsteczkowa

25.9 kDa

opakowanie

pkg of 1 μmol

metody

cell culture | mammalian: suitable

pH

8

przydatność

suitable for

obecność zanieczyszczeń

Trypsin ≤50 BAEE units/mg protein

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Zastosowanie

Elastase from porcine pancreas is used for the following applications:
  • Cell culture techniques
  • ELISA techniques
  • Used in assay for elastase inhibitory activity

Działania biochem./fizjol.

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Opakowanie

Package size based on protein content

Definicja jednostki

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
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Piktogramy

Health hazardExclamation mark

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

P Hamet et al.
The Journal of biological chemistry, 264(21), 12364-12369 (1989-07-25)
The induction of cyclic GMP formation in target tissues, i.e. vascular smooth muscle and endothelial cells, by atrial natriuretic factor is followed by its egression into plasma and urine. Since the extracellular appearance of this cyclic nucleotide is used as
F Seibold et al.
Gut, 32(10), 1192-1197 (1991-10-01)
The presence of antibodies against pancreatic juice (PAB) in patients with Crohn's disease has recently been reported. In our study sera from 273 patients with inflammatory bowel disease (222 with Crohn's disease, 51 with ulcerative colitis) have been examined for
Yoshiyuki Okumura et al.
Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology, 48(1), 13-18 (2007-02-09)
The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid
Adrien Lupieri et al.
Cardiovascular research, 116(2), 438-449 (2019-05-21)
Defects in efficient endothelial healing have been associated with complication of atherosclerosis such as post-angioplasty neoatherosclerosis and plaque erosion leading to thrombus formation. However, current preventive strategies do not consider re-endothelialization in their design. Here, we investigate mechanisms linking immune
Hao Li et al.
Nature communications, 11(1), 3218-3218 (2020-06-27)
Unruptured intracranial aneurysm (UIA) is a life-threatening cerebrovascular condition. Whether changes in gut microbial composition participate in the development of UIAs remains largely unknown. We perform a case-control metagenome-wide association study in two cohorts of Chinese UIA patients and control

Protokoły

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Test enzymatyczny elastazy (EC 3.4.21.36), ciągłe spektrofotometryczne oznaczanie szybkości.

Chromatograms

application for HPLC

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