823428

TOYOPEARL^® AF-rProtein A HC-650F Bulk Media

phase rProtein A, bottle of 1 L, 45 μm particle size

• Synonim(y): TOYOPEARL^® AF-rProtein A HC-650F Bulk Media
• Kod UNSPSC: 41106500

Właściwości

• opis: Adsorption capacity (> 80 g/L IgG (static); >70 g/L IgG (dynamic at 5 min. residence time)); Clean in Place (CIP) (IIID clarifierID clarifier; ID clarif; ierID clarifier0.1); Phase filter (Protein A)
• linia produktu: TOYOPEARL^®
• Postać: slurry
• opakowanie: bottle of 1 L
• producent / nazwa handlowa: Tosoh 23428
• Parametry: 3 bar max. pressure
• metody: LPLC: suitable
• macierz: hydroxylated methacrylic polymer particle platform
• grupa aktywna macierzy: rProtein A phase
• wielkość cząstki: 45 μm
• wielkość porów: 1000 Å
• pH: 2-13
• pojemność: >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time); >80 g/L adsorption capacity (IgG)(static)
• metoda separacji: affinity
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

TOYOPEARL AF-rProtein A HC-650F is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH.
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.

Charakterystyka techniczna

Clean in place with 0.1 M NaOH.

Postać fizyczna

72% (v/v) slurry in 20% (v/v) ethanol

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Informacje prawne

Toyopearl is a registered trademark of Tosoh Corporation

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 3 - Flammable liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): 103.1 °F
• Temperatura zapłonu (°C): 39.5 °C