567508-U
Discovery® HS F5 HPLC Column
5 μm particle size, L × I.D. 5 cm × 2.1 mm
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About This Item
Kod UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Materiały
stainless steel column
Poziom jakości
agency
suitable for USP L43
linia produktu
Discovery®
Właściwości
endcapped
producent / nazwa handlowa
Discovery®
opakowanie
1 ea of
zakres etykietowania
12% Carbon loading
Parametry
≤70 °C temp. range
400 bar pressure (5801 psi)
metody
HPLC: suitable
LC/MS: suitable
dł. × śr. wewn.
5 cm × 2.1 mm
powierzchnia
300 m2/g
pokrycie powierzchni
4 μmol/m2
zanieczyszczenia
<10 ppm metals
macierz
silica gel, high purity, spherical particle platform
fully porous particle
grupa aktywna macierzy
PFP (pentafluorophenyl) phase
wielkość cząstki
5 μm
wielkość porów
120 Å
operating pH range
2-8
Zastosowanie
food and beverages
metoda separacji
reversed phase
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Opis ogólny
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Cechy i korzyści
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Informacje prawne
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Milan Nobilis et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(32), 3845-3852 (2011-11-22)
New bioanalytical SPE-HPLC-PDA-FL method for the determination of the neuroleptic drug tiapride and its N-desethyl metabolite was developed, validated and applied to xenobiochemical and pharmacokinetic studies in humans and animals. The sample preparation process involved solid-phase extraction of diluted plasma
Hiroyuki Kataoka et al.
Analytical and bioanalytical chemistry, 405(1), 331-340 (2012-10-16)
We have developed a simple and sensitive method for the simultaneous determination of testosterone (TES), cortisol (CRT), and dehydroepiandrosterone (DHEA) in saliva by automated online in-tube solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Discovery HS
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
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