ZMS1199

Anti-PSGL-1/CD162 Antibody, clone KPL-1 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonim(y): P-selectin glycoprotein ligand 1, PSGL-1 Selectin P ligand
• Kod UNSPSC: 12352203
• NACRES: NA.43
• białko sprzężone: unconjugated
• application: FACS; ICC; IHC; WB
• klon: KPL-1, recombinant monoclonal
• reaktywność gatunkowa: human
• metody: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• rekombinowane: expressed in HEK 293 cells
• białko sprzężone: unconjugated
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: KPL-1, recombinant monoclonal
• opis: recombinant, expressed in HEK 293 cells
• linia produktu: ZooMAb^® learn more
• Formularz: lyophilized
• oczyszczone przez: using protein G
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 25 μL
• charakterystyka ekologicznej alternatywy: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• rozszerzona walidacja: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• metody: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG1κ
• sekwencja epitopowa: Extracellular domain
• numer dostępu Protein ID: NM_003006.3
• numer dostępu UniProt: Q14242
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specyficzność

Clone KPL-1 is a ZooMAb^® mouse monoclonal antibody that detects PSGL-1/CD162. It targets an epitope within the extracellular domain.

Immunogen

PSGL-1 transfected murine 300.19 pre B-cells.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in HL-60 cell lysates.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PSGL-1/CD162 in HL-60 cell lysates.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PSGL-1/CD162 in lysate from Human peripheral blood mononuclear cells (PBMC)..

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PSGL-1/CD162 in Human tonsil tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected PSGL-1/CD162 in one million Human peripheral blood mononuclear cells (PBMC).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PSGL-1/CD162 in Jurkat cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Opis wartości docelowych

P-selectin glycoprotein ligand 1 (UniProt: Q14242; also known as PSGL-1 Selectin P ligand, CD162) is encoded by the SELPLG gene (Gene ID: 6404) in human. Leukocytes Express^™ L-selectin ligands that are critical for leukocyte-leukocyte interactions at sites of inflammation. PSGL-1 is a disulfide-linked, homodimeric protein. It serves as a predominant leukocyte L-selectin ligand that displays appropriate sialyl Lewis x (sLex)-like carbohydrate determinants for L-selectin recognition. Its expression is observed in neutrophils, monocytes, and most lymphocytes. It is constitutively expressed on all human peripheral-blood T cells and is also expressed on skin-homing T cells. It is a single-pass, mucin-like type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce a form that contains an extracellular domain (18-320), a transmembrane domain (321-341), and a cytoplasmic domain (342-412). The mature form is generated following the cleaved of the propeptide (aa 18-41) region. PSGL-1 is a SLe(x)-type proteoglycan, which through high Affinity^™, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Two isoforms of PSGL-1/CD162 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals.  (Ref.: Tu, L., et al. (1999). J. Immunol. 163(9); 5070-5078; Fuhlbrigge, RC., et al. (1997). Nature. 389(6654); 978-981; Duivestijn, AM., et al. (1988). Am. J. Pathol. 130(1); 147-155).

Postać fizyczna

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstytucja

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

Affinity is a trademark of Mine Safety Appliances Co.

Express is a trademark of Merck KGaA, Darmstadt, Germany

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable