MAB4092

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

clone KPL-1, Chemicon^®, from mouse

• Synonim(y): PSGL-1, CD162
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: KPL-1, monoclonal
• reaktywność gatunkowa: human
• spodziewany brak reakcji z: rat (Snapp, KR et al 1998; Baisse, B et al 2007), pig (Snapp, KR et al 1998; Baisse, B et al 2007), bovine (Snapp, KR et al 1998; Baisse, B et al 2007), equine (Snapp, KR et al 1998; Baisse, B et al 2007)
• producent / nazwa handlowa: Chemicon^®
• metody: flow cytometry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NM_003006.3
• numer dostępu UniProt: Q14242
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SELPLG(6404)

Opis

Opis ogólny

Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.

Specyficzność

P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.

Immunogen

The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).

Zastosowanie

Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000

FACS {Snapp, KR et al 1998}.

Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).

Functional blocking of P-selectin {Snapp KR et al 1998}.

Immunohistochemistry: Fresh frozen, acetone fixed sections

Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200

Optimal working dilutions must be determined by the end user.

Postać fizyczna

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.

Przechowywanie i stabilność

Maintain at 2-8°C in undiluted aliquots up to 12 months.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable