T9784

Tricine

BioXtra, pH 4.0-6.0 (1 M in H₂O), ≥99% (titration)

• Synonim(y): N-[Tris(hydroxymethyl)methyl]glycine
• Wzór liniowy: (HOCH₂)₃CNHCH₂CO₂H
• Numer CAS: 5704-04-1
• Masa cząsteczkowa: 179.17
• Beilstein: 1937804
• Numer WE: 227-193-6
• Numer MDL: MFCD00004277
• Kod UNSPSC: 12161700
• Identyfikator substancji w PubChem: 24900603
• NACRES: NA.25

Właściwości

• linia produktu: BioXtra
• Poziom jakości: 400
• Próba: ≥99% (titration)
• Formularz: powder
• zanieczyszczenia: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• pozostałość po prażeniu: ≤0.1%
• pH: 4.0-6.0 (1 M in H₂O)
• przydatny zakres pH: 7.4-8.8
• pKa (25°C): 8.1
• rozpuszczalność: H₂O: 1 M, clear, colorless
• ślady anionów: chloride (Cl^-): ≤0.1%; sulfate (SO₄^2-): ≤0.05%
• ślady kationów: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• Absorpcja: ≤0.05 at 280 in H₂O at 1 M; ≤0.1 at 260 in H₂O at 1 M
• Zastosowanie: diagnostic assay manufacturing
• ciąg SMILES: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• Klucz InChI: SEQKRHFRPICQDD-UHFFFAOYSA-N

Opis

Opis ogólny

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

Zastosowanie

Tricine has been used:

• in a polymerase chain reaction (PCR) buffer used in the single-cell collection
• as a component of lactotroph feeding medium for culturing lactotrophs
• to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer

Buffer component for separation of low molecular weight peptides.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)