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Key Documents

SRP3200

Sigma-Aldrich

GDNF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonim(y):

ATF-1, Glial-Derived Neurotrophic Factor

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About This Item

Kod UNSPSC:
12352202
NACRES:
NA.32

pochodzenie biologiczne

mouse

rekombinowane

expressed in E. coli

Próba

≥98% (HPLC)
≥98% (SDS-PAGE)

Postać

lyophilized

siła działania

≤0.2 ng/mL

masa cząsteczkowa

15.1 kDa

opakowanie

pkg of 10 μg

metody

cell culture | mammalian: suitable

zanieczyszczenia

<0.1 EU/μg endotoxin, tested

kolor

white to off-white

numer dostępu UniProt

Warunki transportu

wet ice

temp. przechowywania

−20°C

informacje o genach

mouse ... GDNF(14573)

Opis ogólny

GDNF (glial cell line derived neurotrophic factor) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Zastosowanie

GDNF from mouse has been used for stimulating primary murine cultures of dorsal root ganglion (DRG) neurons.
GDNF from mouse has been used to study the role of NEDL2 (Nedd4-related E3 ubiquitin ligase-2) in GDNF/Ret signaling.

Działania biochem./fizjol.

GDNF (glial cell line derived neurotrophic factor) signals through a multicomponent receptor system, composed of a RET (receptor tyrosine kinase) and one of the four GFR-α (α1-α4) receptors (GDNF family receptors). GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson′s disease monkey model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. It might also be involved in the treatment of non-alcoholic fatty liver disease. GDNF is needed for proper spermatogonial stem cell survival and proliferation.

Sekwencja

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Postać fizyczna

Lyophilized from 10 mM Sodium Citrate.

Rekonstytucja

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Peripheral expression and biological activities of GDNF, a new neurotrophic factor for avian and mammalian peripheral neurons.
Trupp M, et al.
The Journal of Cell Biology, 130, 137-148 (1995)
Functional recovery in parkinsonian monkeys treated with GDNF.
Gash DM, et al.
Nature, 380, 252-255 (1996)
A rapid induction mechanism for Lin28a in trophic responses
Amen Am, et al.
Molecular Cell, 65(3), 490-503 (2017)
Peritubular myoid cells participate in male mouse spermatogonial stem cell maintenance.
Chen LY, et al.
Endocrinology, 155, 4964-4974 (2014)
NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling.
Wei R, et al.
Cellular Signalling, 27, 578-586 (2015)

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