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B3795
Brain-derived neurotrophic factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonim(y):
Abrineurin, BDNF
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About This Item
Polecane produkty
Nazwa produktu
Brain-derived neurotrophic factor human, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in E. coli
Próba
≥95% (SDS-PAGE)
Formularz
lyophilized powder
jakość
endotoxin tested
masa cząsteczkowa
~27 kDa
opakowanie
pkg of 10 μg
pkg of 5 μg
warunki przechowywania
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤1.00 EU/μg Endotoxin level
numer dostępu UniProt
temp. przechowywania
−20°C
Opis ogólny
BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
Zastosowanie
Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.
Działania biochem./fizjol.
Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.
Postać fizyczna
lyophilized from a sterile 0.2 μm filtered solution with BSA
Komentarz do analizy
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Protokoły
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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