SRP0132

EZH2 / EED / SUZ12 human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Synonim(y): ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
• Kod UNSPSC: 12352200
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: human
• rekombinowane: expressed in baculovirus infected insect cells
• Próba: ≥90% (SDS-PAGE)
• Postać: aqueous solution
• masa cząsteczkowa: 51 kDa (EED); 86 kDa (EZH2); 87 kDa (SUZ12)
• opakowanie: pkg of 50 μg
• stężenie: >0.02 mg/mL
• numer dostępu NCBI: NM_003797 (EED); NM_004456 (EZH2); NM_015355 (SUZ12)
• numer dostępu UniProt: Q15910
• Warunki transportu: dry ice
• temp. przechowywania: −70°C
• informacje o genach: human ... EZH2(2146)

Opis

Opis ogólny

Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW = 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag.

Zastosowanie

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definicja jednostki

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Postać fizyczna

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.

Uwaga dotycząca przygotowania

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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