SAB3700670

Anti-Rat IgM (μ-chain specific) antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Kod UNSPSC: 12352203
• NACRES: NA.46

Właściwości

• pochodzenie biologiczne: goat
• białko sprzężone: unconjugated
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: secondary antibodies
• klon: polyclonal
• Postać: buffered aqueous solution
• reaktywność gatunkowa: rat
• stężenie: 1.0 mg/mL
• metody: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

Immunoglobulin M (IgM) belongs to the immunoglobulin family. It exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten μ-heavy chains, ten light chains and a single J-chain which are linked by disulphide bonds. The μ-chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The heavy chains of IgM contain an extra C domain that replaces the hinge region present between Fab and Fc. IgM is the first antibody to be produced during an immune response and is the major isotype secreted in T-cell independent immune responses.

Specyficzność

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgG or Rat IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other rat heavy or light chain isotypes.

Immunogen

Rat IgM mu heavy chain

Właściwości fizyczne

Antibody format: IgG

Postać fizyczna

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable