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S8409
Superoxide Dismutase from bovine liver
ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)
Synonim(y):
SOD, Superoxide: superoxide oxidoreductase
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About This Item
Polecane produkty
Formularz
ammonium sulfate suspension
Poziom jakości
aktywność właściwa
2,000-6,000 units/mg protein (biuret)
masa cząsteczkowa
32.5 kDa
numer dostępu UniProt
temp. przechowywania
2-8°C
informacje o genach
cow ... SOD1(281495)
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Opis ogólny
Research area: Cell Signaling
Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals, and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.
Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals, and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.
Zastosowanie
Superoxide Dismutase from bovine liver has been used:
- for measuring mitochondrial hydrogen peroxide production in skeletal muscle
- as a positive control in Cu(II) binding stability assay
- as a positive control in superoxide dismutase assay
- in a study to assess the inactivation of endothelial-derived relaxing factor by oxidized lipoproteins.
- in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.
Działania biochem./fizjol.
Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.
Definicja jednostki
One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Postać fizyczna
Suspension in 3.8 M (NH4)2SO4, pH 7.0
Komentarz do analizy
For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).
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Zastosowanie
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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E M Link et al.
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1. The survival of mammalian epithelial cells exposed in vitro to the xanthine/xanthine oxidase system in phosphate-buffered saline (PBS) or serum-containing medium (SCMEM) was investigated. 2. The cytotoxic effect observed depended on the composition of the medium in which the
L G Chi et al.
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Produkty
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Protokoły
Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
Chromatograms
application for HPLCNasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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