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S9636
Superoxide Dismutase from human erythrocytes
essentially salt-free, lyophilized powder, ≥2,500 units/mg protein
Synonim(y):
SOD, Superoxide: superoxide oxidoreductase
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About This Item
Polecane produkty
pochodzenie biologiczne
human erythrocytes
Poziom jakości
Próba
>80% protein (biuret)
Formularz
essentially salt-free, lyophilized powder
aktywność właściwa
≥2,500 units/mg protein
masa cząsteczkowa
32.0 kDa
skład
Protein, ≥80% biuret
producent / nazwa handlowa
Sigma-Aldrich
metody
activity assay: suitable
kolor
white to off-white
zakres pH
7.6—10.5
pH
7.8
przydatność
suitable for molecular biology
Zastosowanie
life science and biopharma
temp. przechowywania
−20°C
informacje o genach
human ... SOD1(6647) , SOD2(6648) , SOD3(6649)
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Opis ogólny
Superoxide dismutases (SOD) are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. Three forms of SOD exist, based on the metal ions in the active site. These are Cu2+/Zn2+, Mn2+ and Fe2+ containing SOD. In vertebrate organisms, Cu/Zn-SOD is located in the cytoplasm as well as the mitochondrial intermembrane space, whereas Mn-SOD is located at the mitochondrial matrix space in prokaryotes. Fe-SOD is also found in prokaryotes and higher plants. Human erythrocyte SOD is a non-covalently bound homodimeric protein with two 16.3 kDa subunits containing 153 amino acids. Each dimer consists of two Cu2+ atoms and two Zn2+ atoms.
Zastosowanie
Superoxide Dismutase from human erythrocytes has been used:
- to test its effect on human neutrophils in reactive oxygen species (ROS) measurement studies involving Pseudomonas aeruginosa infection
- as an antioxidant to test its effect on ROS generation induced by atmospheric-pressure plasma jet (APPJ) in red blood cell (RBC) homogenates using optical spectroscopy studies
- to test its attenuating effect on hemoglobin (Hb)-induced nuclear factor-kappa B (NF- κB) and hypoxia-inducible factor (HIF) activity in human dermal microvascular endothelial cells (HMECs-1)
- as a reference antioxidant protein to examine its expression in human intestinal Caco-2 cells following treatment with dietary flavonoids
- in combination with catalase to promote cell differentiation in vitro
Działania biochem./fizjol.
Mutations in the SOD1 gene are implicated in Amyotrophic lateral sclerosis (ALS).
Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.
Definicja jednostki
One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Komentarz do analizy
For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Produkty
Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.
Protokoły
Enzymatic Assay of Superoxide Dismutase
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