S7571

Superoxide Dismutase from bovine erythrocytes

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

• Synonim(y): CU/ZN-SOD, Superoxide Dismutase 1 bovine, cytocuprein, erythrocuprein, hemocuprein, SOD, Superoxide: superoxide oxidoreductase
• Numer CAS: 9054-89-1
• Numer EC enzymu: 1.15.1.1 (BRENDA, IUBMB)
• Numer WE: 232-943-0
• Numer MDL: MFCD00132404
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: bovine
• Poziom jakości: 300
• Formularz: lyophilized powder
• aktywność właściwa: ≥3,000 units/mg protein
• masa cząsteczkowa: 32.5 kDa
• skład: Protein, ≥95% biuret
• warunki przechowywania: (Store under nitrogen.; Tightly closed. Dry.)
• charakterystyka ekologicznej alternatywy: Atom Economy; Design for Energy Efficiency; Use of Renewable Feedstocks; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• metody: immunoblotting: suitable; inhibition assay: suitable
• kolor: blue-green
• pI: 4.95
• rozpuszczalność: water: 20 mg/mL; aqueous buffer, pH 7.5: soluble
• numer dostępu UniProt: P00442; P41976
• Zastosowanie: diagnostic assay manufacturing
• kategoria ekologicznej alternatywy: , Re-engineered
• temp. przechowywania: −20°C
• informacje o genach: cow ... SOD1(281495) , SOD2(281496)

Opis

Opis ogólny

Research area: Cell Signaling

Superoxide dismutase (SOD) is a redox-active metalloenzyme expressed in both aerobic and anaerobic living organisms. Bovine superoxide dismutase or CuZn SOD is a homodimer with each subunit containing one zinc and one copper ion.

Zastosowanie

Superoxide dismutase from bovine erythrocytes has been used:

• in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions
• in a study to investigate the possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction
• to investigate its effect on the hemolysis rate of human RBCs and hemoglobin-nitric oxide complex (HbNO) stability in human erythrocytes
• in combination with catalase to study its effect on cell differentiation in vitro
• to quantify superoxide levels and study their effect on reactivity in mouse pulmonary arteries through chemiluminescence and cytochrome C reduction methods

Działania biochem./fizjol.

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Definicja jednostki

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Postać fizyczna

Lyophilized powder, essentially salt-free

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)