SRE0041

Catalase from bovine liver

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 2,000-5,000 units/mg protein

• Synonim(y): Catalase from bovine liver, H₂O₂:H₂O₂ oxidoreductase
• Numer CAS: 9001-05-2
• Numer EC enzymu: 1.11.1.6 (BRENDA, IUBMB)
• Numer WE: 232-577-1
• Numer MDL: MFCD00081483
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• Poziom jakości: 400
• Postać: lyophilized powder
• aktywność właściwa: 2,000-5,000 units/mg protein
• masa cząsteczkowa: tetramer ~250 kDa
• punkt izoelektryczny: 5.4
• Zastosowanie: diagnostic assay manufacturing
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• Klucz InChI: NUVBSKCKDOMJSU-UHFFFAOYSA-N

Opis

Opis ogólny

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Zastosowanie

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

• to prepare H₂O₂-O₂ based biocathode for applications in glucose biofuel cells
• to study the kinetic properties and storage stability of catalase immobilized on to florisil
• in glutathione-mediated superoxide generation in an aqueous solution

Działania biochem./fizjol.

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

Definicja jednostki

One unit will decompose 1.0 micromole of hydrogen peroxide per minute at pH 7.0 and 25 deg C, while the hydrogen peroxide concentration falls from 10.3 to 9.2 millimolar.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable