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P1016
Phenylalanine Ammonia-Lyase from Rhodotorula glutinis
Grade I, buffered aqueous glycerol solution, 0.8-2.0 units/mg protein
Synonim(y):
L-Phenylalanine ammonia-lyase, Phenylalanine Deaminase from Rhodotorula glutinis
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.54
Polecane produkty
pochodzenie biologiczne
fungus (Rhodotorula glutinis)
Poziom jakości
typ
Grade I
Formularz
buffered aqueous glycerol solution
aktywność właściwa
0.8-2.0 units/mg protein
Warunki transportu
wet ice
temp. przechowywania
−20°C
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Zastosowanie
Phenylalanine ammonia-lyase has been used in a study to assess the effect of heat treatment on lignification of postharvest bamboo shoots. It has also been used in a study to investigate the effect of light on gene expression and podophyllotoxin biosynthesis in Linum album cell culture.
Działania biochem./fizjol.
Phenylalanine Ammonia-Lyase from Rhodotorula glutinis can be cleaved and inactivated by the proteases chymotrypsin, subtilisin, and trypsin.
Definicja jednostki
One unit will deaminate 1.0 μmole of L-phenylalanine to trans-cinnamate and NH3 per min at pH 8.5 at 30 °C.
Postać fizyczna
Solution in 60% glycerol, 3 mM Tris-HCl, pH 7.5, containing up to 0.5 M (NH4)2SO4
Komentarz do analizy
Protein determined by biuret
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Inhibitor
Numer produktu
Opis
Cennik
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Phenylalanine ammonia-lyase (EC 4.3.1.5) of the yeast Rhodotorula glutinis was rapidly inactivated by duodenal juice. It was susceptible to chymotrypsin and subtilisin and to a lesser extent trypsin. Initial proteolysis of the enzyme by chymotrypsin and trypsin resulted in cleavage
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