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S4920
SITE Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonim(y):
Media supplement
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352205
NACRES:
NA.75
Polecane produkty
sterylność
sterile-filtered
Poziom jakości
linia produktu
BioReagent
Formularz
liquid
metody
cell culture | mammalian: suitable
zanieczyszczenia
endotoxin, tested
Warunki transportu
ambient
temp. przechowywania
2-8°C
Opis ogólny
SITE Liquid Media Supplement (100×) serves as a substitute for serum-free formulations. It contains purified factors required for in vitro growth and differentiation studies. The addition of supplements to media will vary, depending on the cell type being studied and the basal medium employed. It is a general cell supplement designed for use in non-complex media (e.g., minimum essential medium (MEM), Roswell Park Memorial Institute Medium (RPMI-1640)) and complex media (e.g., Ham′s F-12, Dulbecco′s Modified Eagle Medium (DME) /F-12, MEM) with sodium pyruvate.
Zastosowanie
SITE Liquid Media Supplement (100×) has been used:
- in the 3D culture of mouse embryonic pancreatic epithelial cells
- to isolate rabbit gastric glands and parietal cells
- to isolate rabbit single proximal tubule cells
Działania biochem./fizjol.
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
- Selenium: an essential trace element normally provided by serum.
- Ethanolamine: plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.
Komponenty
Contains 1.0mg/ml recombinant human insulin, 0.55mg/ml human transferrin (substantially iron-free), 0.5μg/ml sodium selenite and 0.2mg/ml ethanolamine at the 100x concentration.
Uwaga dotycząca przygotowania
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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