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Merck

I3146

Sigma-Aldrich

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonim(y):

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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About This Item

Kod UNSPSC:
12352205
NACRES:
NA.75

sterylność

sterile-filtered

Poziom jakości

linia produktu

BioReagent

Postać

liquid

metody

cell culture | mammalian: suitable
single cell analysis: suitable

zanieczyszczenia

endotoxin, tested

Warunki transportu

ambient

temp. przechowywania

2-8°C

Zastosowanie

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells
  • rat fetal testis explants
  • human dental pulp stem cells (DPSC)

Działania biochem./fizjol.

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.

Komponenty

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Inne uwagi

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Uwaga dotycząca przygotowania

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

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Produkty

Poznaj rolę selenu w formułach pożywek do hodowli komórkowych. Biblioteka Media Expert wyjaśnia, w jaki sposób składniki pożywek hodowlanych wspierają wzrost komórek.

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.

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