11074547001
Roche
Suplement insulina-transferryna-selenit sodu
suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium)
Synonim(y):
ITS
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About This Item
Kod UNSPSC:
12352207
Polecane produkty
pochodzenie biologiczne
human serum (transferrin)
synthetic (sodium selenite)
Poziom jakości
rekombinowane
expressed in human cells
sterylność
non-sterile; 0.2 μm filtered
Próba
>98% (Transferrin, SDS-PAGE)
>99% (Insulin, HPLC)
>99% (Sodium selenite)
Formularz
lyophilized
opakowanie
pkg of 50 mg (for 5 l medium)
producent / nazwa handlowa
Roche
metody
cell culture | mammalian: suitable
rozpuszczalność
water: miscible
temp. przechowywania
2-8°C
informacje o genach
bovine ... INS(280829)
human ... TFRC(7037)
Opis ogólny
The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.
Zastosowanie
The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.
Uwaga dotycząca przygotowania
Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
Rekonstytucja
The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.
Further dilution with culture medium.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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