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P7255

Sigma-Aldrich

Puromycin dihydrochloride from Streptomyces alboniger

greener alternative

≥98% (HPLC), powder

Synonim(y):

3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

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About This Item

Wzór empiryczny (zapis Hilla):
C22H29N7O5 · 2HCl
Numer CAS:
58-58-2
Masa cząsteczkowa:
544.43
Beilstein:
3853613
Numer WE:
200-387-8
Numer MDL:
MFCD00012691
Kod UNSPSC:
51281912
Identyfikator substancji w PubChem:
57654556
NACRES:
NA.77

Poziom jakości

300

Próba

≥98% (HPLC)

Postać

powder

wynik ekologicznej alternatywy

old score: 88
new score: 79
Find out more about DOZN™ Scoring

charakterystyka ekologicznej alternatywy

Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
Design for Degradation
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

rozpuszczalność

H2O: 50 mg/mL

spektrum działania antybiotyku

Gram-positive bacteria
neoplastics
parasites

kategoria ekologicznej alternatywy

, Re-engineered

Tryb działania

protein synthesis | interferes

temp. przechowywania

−20°C

ciąg SMILES

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

Klucz InChI

MKSVFGKWZLUTTO-FZFAUISWSA-N

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Opis ogólny

Chemical structure: peptidyl nucleoside
Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.
We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Atom Economy”, “Design for Energy Efficiency”, “Use of Renewable Feedstock” and “Design for Degradation”. Click here to view its DOZN scorecard.

Zastosowanie

Recommended for use as a selection agent at a range of 1-10 μg/mL.

Działania biochem./fizjol.

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.
Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Cechy i korzyści

This compound is a featured product for Gene Regulation research. Click here to discover more featured Gene Regulation products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Przestroga

As supplied, this product remains active for four years when stored at -20°C.

Uwaga dotycząca przygotowania

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

Inne uwagi

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place. Keep in a dry place
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Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H302

Zwroty wskazujące środki ostrożności

P301 + P312 + P330

Klasyfikacja zagrożeń

Acute Tox. 4 Oral

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Gloves

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Claudia Fritsch et al.
Genome research, 22(11), 2208-2218 (2012-08-11)
So far, the annotation of translation initiation sites (TISs) has been based mostly upon bioinformatics rather than experimental evidence. We adapted ribosomal footprinting to puromycin-treated cells to generate a transcriptome-wide map of TISs in a human monocytic cell line. A
Robert J Huber et al.
Biochimica et biophysica acta, 1833(1), 11-20 (2012-10-16)
Cyclin-dependent kinase 5 (Cdk5) is a serine/threonine kinase that has been implicated in a number of cellular processes. In Dictyostelium, Cdk5 localizes to the nucleus and cytoplasm, interacts with puromycin-sensitive aminopeptidase A (PsaA), and regulates endocytosis, secretion, growth, and multicellular
Shingo Ueno et al.
Journal of biotechnology, 162(2-3), 299-302 (2012-09-25)
cDNA display using a puromycin-linker to covalently bridge a protein and its coding cDNA is a stable and efficient in vitro protein selection method. The optimal design of the often-used puromycin-linker is vital for effective selection. In this report, an
Robert A J Signer et al.
Nature, 509(7498), 49-54 (2014-03-29)
Many aspects of cellular physiology remain unstudied in somatic stem cells, for example, there are almost no data on protein synthesis in any somatic stem cell. Here we set out to compare protein synthesis in haematopoietic stem cells (HSCs) and
Bence Daniel et al.
Immunity, 49(4), 615-626 (2018-10-18)
Macrophages polarize into distinct phenotypes in response to complex environmental cues. We found that the nuclear receptor PPARγ drove robust phenotypic changes in macrophages upon repeated stimulation with interleukin (IL)-4. The functions of PPARγ on macrophage polarization in this setting
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Produkty

Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere with the processes at the 30S subunit or 50S subunit of the 70S bacterial ribosome.

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