A1720

G 418 disulfate salt

powder, non-animal origin, suitable for cell culture, BioReagent

• Synonim(y): Antibiotic G418
• Wzór empiryczny (zapis Hilla): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• Numer CAS: 108321-42-2
• Masa cząsteczkowa: 692.71
• Numer MDL: MFCD00058314
• Kod UNSPSC: 12352207
• Identyfikator substancji w PubChem: 24890581
• NACRES: NA.76

Właściwości

• product name: G 418 disulfate salt, powder, BioReagent, suitable for cell culture
• linia produktu: BioReagent
• Postać: powder
• aktywność optyczna: [α]/D (Specific Rotation: +104.4^o (c=0.3% in H₂O at 26^oC))
• siła działania: ≥720 μg per mg (Dried basis)
• masa cząsteczkowa: 692.7
• opakowanie: pkg of 1 g; pkg of 25 g; pkg of 5 g
• metody: cell culture | mammalian: suitable
• kolor: white to off-white
• rozpuszczalność: H₂O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)
• spektrum działania antybiotyku: Gram-negative bacteria
• Tryb działania: protein synthesis | interferes
• temp. przechowywania: 2-8°C
• ciąg SMILES: OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• Klucz InChI: UHEPSJJJMTWUCP-NKCAIAFTSA-N

Opis

Opis ogólny

Chemical structure: aminoglycoside

G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.

Zastosowanie

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Działania biochem./fizjol.

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.

Przestroga

The product is stable for two years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

Uwaga dotycząca przygotowania

G418 powder is soluble in water at 100 mg/mL.

Inne uwagi

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H317,H334
• Zwroty wskazujące środki ostrożności: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)